caco 2 (ATCC)
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caco 2
![( A ) Timeline of MGCAoC <t>establishment.</t> <t>Caco-2</t> cells were seeded in the gut channel and cultured under dynamic flow for intestinal epithelium formation over 5 days, which was followed by the seeding of probiotics. BMSC suspension was injected into the microwell array for self-aggregation and chondrogenic differentiation. After 2 days of chondrogenic induction, the cartilage module was connected to the microbe-gut module by adjusting the embedded microvalves to establish the MGCAoC. BB , B. bifidum ; LP , L. plantarum. ( B ) Quantification of apparent permeability of intestinal epithelial monolayers over 7 days [ n = 3 chips, one-way analysis of variance (ANOVA)]. ( C ) Transepithelial resistance (TEER) values of intestinal epithelial monolayers formed in the gut module of MGCAoC over 7 days ( n = 3 chips, one-way ANOVA). d, day. ( D ) Scanning electron microscopy (SEM) image showing the intestinal villus-crypt structures on the established Caco-2 epithelium. Scale bar, 1 μm. ( E ) SEM images of the apical surface of probiotic-seeded intestinal epithelium. The right image shows a magnified view of the area in the white dashed box, where a probiotic community is observed on the mucus. The probiotics were seeded on day 5, and the images were taken 2 days afterward. Scale bars, 1 μm. ( F ) Confocal images showing ZO-1 expression (green) and BB and LP distribution (red) on the intestinal epithelium. ( G ) Confocal images showing the relative positions of the intestinal epithelial layer (blue), the mucus layer (green), and probiotics (red). ( H ) Bright-field images of COs cultured on 96-well plates, CO-on-a-chip (COoC; probiotic-free), and MGCAoC platform. The images were taken on day 14. Scale bar, 200 μm. ( I ) Changes in CO diameters after culturing on 96-well plates, COoC (probiotic-free), and MGCAoC platform for different durations ( n = 10, two-way ANOVA). d, day. ( J ) Terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick end labeling (TUNEL) staining of CO sections, with red color indicating apoptotic cells. Scale bars, 200 μm. ( K ) Percentage of TUNEL-positive cells ( n = 5, one-way ANOVA). n.s., not significant.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_1862/pmc13651862/pmc13651862__sciadv.aeg8557-f3.jpg)
Caco 2, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 15317 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/caco+2/Caco-2/pmc13651862-236-4-21
Average 99 stars, based on 15317 article reviews
Caco 2, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 15317 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/caco+2/Caco-2/pmc13651862-236-4-21
Average 99 stars, based on 15317 article reviews
caco 2 - by Bioz Stars,
2026-10
99/100 stars
Images
1) Product Images from "Effects of probiotics on joint homeostasis and osteoarthritis: Development of microbe-gut-cartilage axis-on-a-chip"
Article Title: Effects of probiotics on joint homeostasis and osteoarthritis: Development of microbe-gut-cartilage axis-on-a-chip
Journal: Science Advances
doi: 10.1126/sciadv.aeg8557
Figure Legend Snippet: ( A ) Timeline of MGCAoC establishment. Caco-2 cells were seeded in the gut channel and cultured under dynamic flow for intestinal epithelium formation over 5 days, which was followed by the seeding of probiotics. BMSC suspension was injected into the microwell array for self-aggregation and chondrogenic differentiation. After 2 days of chondrogenic induction, the cartilage module was connected to the microbe-gut module by adjusting the embedded microvalves to establish the MGCAoC. BB , B. bifidum ; LP , L. plantarum. ( B ) Quantification of apparent permeability of intestinal epithelial monolayers over 7 days [ n = 3 chips, one-way analysis of variance (ANOVA)]. ( C ) Transepithelial resistance (TEER) values of intestinal epithelial monolayers formed in the gut module of MGCAoC over 7 days ( n = 3 chips, one-way ANOVA). d, day. ( D ) Scanning electron microscopy (SEM) image showing the intestinal villus-crypt structures on the established Caco-2 epithelium. Scale bar, 1 μm. ( E ) SEM images of the apical surface of probiotic-seeded intestinal epithelium. The right image shows a magnified view of the area in the white dashed box, where a probiotic community is observed on the mucus. The probiotics were seeded on day 5, and the images were taken 2 days afterward. Scale bars, 1 μm. ( F ) Confocal images showing ZO-1 expression (green) and BB and LP distribution (red) on the intestinal epithelium. ( G ) Confocal images showing the relative positions of the intestinal epithelial layer (blue), the mucus layer (green), and probiotics (red). ( H ) Bright-field images of COs cultured on 96-well plates, CO-on-a-chip (COoC; probiotic-free), and MGCAoC platform. The images were taken on day 14. Scale bar, 200 μm. ( I ) Changes in CO diameters after culturing on 96-well plates, COoC (probiotic-free), and MGCAoC platform for different durations ( n = 10, two-way ANOVA). d, day. ( J ) Terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick end labeling (TUNEL) staining of CO sections, with red color indicating apoptotic cells. Scale bars, 200 μm. ( K ) Percentage of TUNEL-positive cells ( n = 5, one-way ANOVA). n.s., not significant.
Techniques Used: Cell Culture, Probiotics, Suspension, Injection, Permeability, Analysis, Scanning Electron Microscopy, Confocal, Expressing, 96 Well Plates, End Labeling, TUNEL Assay, Staining
Figure Legend Snippet: ( A ) Timeline of metabolomics analysis. Caco-2 cells were seeded in the gut channel and cultured under dynamic flow for intestinal epithelium formation over 5 days, which was followed by seeding of the probiotics. During sample collection, μV2 in the inner circulation channel was closed, allowing the effluent to exit the chip through the open μV1. Created in BioRender. J. Liu (2026) https://BioRender.com/yxqe60a . ( B ) Principal components analysis (PCA) plot and ( C ) orthogonal partial least-squares discriminant analysis (OPLS-DA) plot for metabolomics analysis of gut module effluent from chips with (MGCAoC) or without (GCAoC) probiotic colonization ( n = 3 chips). ( D ) Volcano plot for differential metabolites in gut module effluent from chips with or without probiotic colonization. FC, fold change. ( E ) Heatmap of significantly up-regulated and down-regulated metabolites. AMP, adenosine 5′-monophosphate. ( F ) Pathway analysis for differentially enriched metabolites. ( G ) Concentrations of representative significantly up-regulated metabolites in gut module effluent from MGCAoC compared to GCAoC ( n = 3 chips, unpaired t test).
Techniques Used: Analysis, Cell Culture, Probiotics
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